Hypoxia-induced myeloid derived growth factor promotes hepatocellular carcinoma progression through remodeling tumor microenvironment

Purpose: Exploring and studying the novel target of hepatocellular carcinoma (HCC) has been extremely important for its treatment. The principal objective this project is to investigate whether myeloid derived growth factor (MYDGF) could accelerate progression HCC, how it works. Methods: Cell proliferation, clonal formation, sphere formation xenograft tumor experiments were used prove critical role MYDGF in HCC progression. Tumor angiogenesis, immune cell infiltration, macrophage chemotaxis inflammatory cytokines detection utilized clarify remodeled microenvironment (TME) progress HCC. Results: Here, we reported a secretory protein MYDGF, which be induced by hypoxia, was significantly upregulated associated with poor clinical outcomes. Using bioinformatics experimental approaches, found that promotes proliferation vitro vivo through mechanism might involve enhanced self-renewal liver CSCs. Furthermore, can also promote induce macrophages into tissue, then release various cytokines, including IL-6 TNF-?, ultimately aggravate inflammation Conclusions: We provided evidence directly affect CSCs, indirectly